Anti-MuLVs Monoclonal Antibodies

PA007641.r2a: In Vivo Grade Recombinant Anti-MuLVs Monoclonal Antibody (Clone: 83A25), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.
Clone: 83A25.
Isotype: Rat IgG2a kappa.
Source: The anti-MuLVs monoclonal antibody (clone: 83A25) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 83A25) specifically binds to MuLVs.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the MuLVs protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-MuLVs monoclonal antibody of clone 83A25 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-MuLVs antibody (Clone: 83A25):

Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line
Lusso P, et al. Retrovirology. 2008 Jan 3;5:1. doi: 10.1186/1742-4690-5-1. PMID: 18182012
Cell surface expression of MuLV gp70 protein (SU) was revealed by immunofluorescence assay using the broadly reactive monoclonal antibody 83A25. Newborn mice developed lymphoma following inoculation with the MuLV mixture expressed by these cells. As currently available, however, we find that it produces significant levels of ecotropic MuLV with the biologic features of the Moloney isolate and also MuLV of the polytropic or MCF class. To look for expression of MuLV-encoded protein, RAW264.7 cell pellets were fixed in formalin and embedded in paraffin; after antigen retrieval using proteinase K, sections were stained by avidin-biotin immunohistochemistry (IHC) using group-reactive goat anti-Rauscher MuLV p30 antibody. Abundant expression of MuLV p30 was revealed.
Tags: anti-MuLVs 83A25; anti-MuLVs 83A25 antibody

Mouse APOBEC3 restricts friend leukemia virus infection and pathogenesis in vivo
Takeda E, et al. J Virol. 2008 Nov;82(21):10417-25. doi: 10.1128/JVI.01389-08. PMID: 18786991
Infection of cell cultures of various mouse strains by most ecotropic MuLVs is regulated by the Fv1 genotype of the mouse and the tropism of the virus. Here we have shown that polymorphisms at the mouse APOBEC3 locus indeed influence F-MuLV replication and pathogenesis: the APOBEC3 alleles of F-MuLV-resistant C57BL/6 and -susceptible BALB/c mice differ in their sequences and expression levels in the hematopoietic tissues and in their abilities to restrict F-MuLV replication both in vitro and in vivo. The pathogenicity of Friend MuLV (F-MuLV) is influenced by several host genes: some directly restrict the cell entry or integration of the virus, while others influence the host immune responses. Although the betaretrovirus mouse mammary tumor virus has been shown to be susceptible to mouse APOBEC3, it is not known if APOBEC3 has a physiological role in restricting more widely distributed and long-coevolved mouse gammaretroviruses. Likewise, murine leukemia viruses (MuLV) exclude mouse APOBEC3 from the virions and cleave virion-incorporated APOBEC3.
Tags: anti-MuLVs 83A25 in vivo; anti-MuLVs 83A25 in animal model

A neutralizable epitope common to the envelope glycoproteins of ecotropic, polytropic, xenotropic, and amphotropic murine leukemia viruses
Evans LH, et al. J Virol. 1990 Dec;64(12):6176-83. doi: 10.1128/JVI.64.12.6176-6183.1990. PMID: 1700832
An epitope common to all classes of murine leukemia viruses (MuLVs) was detected by reactivity of MuLVs with a rat monoclonal antibody (MAb) termed 83A25. The epitope served as a target for neutralization of all classes of MuLV with MAb 83A25. The efficiency of neutralization varied with different MuLV isolates but did not correlate with MuLV interference groups. The antibody is of the immunoglobulin G2a isotype and was derived after fusion of NS-1 myeloma cells with spleen cells from a Fischer rat immunized with a Friend polytropic MuLV. The epitope was localized to the SU portion of the envelope glycoprotein by immunoprecipitation of in vitro translation products of cloned viral env genes.
Tags: anti-MuLVs 83A25 mAb in animal model; anti-MuLVs 83A25 in cancer research

Analysis of the Ex Vivo and In Vivo Antiretroviral Activity of Gemcitabine
Donahue DA, et al. PLoS One. 2011 Jan 13;6(1):e15840. doi: 10.1371/journal.pone.0015840. PMID: 21249250
To look for expression of MuLV-encoded protein, RAW264.7 cell pellets were fixed in formalin and embedded in paraffin; after antigen retrieval using proteinase K, sections were stained by avidin-biotin immunohistochemistry (IHC) using group-reactive goat anti-Rauscher MuLV p30 antibody (from the Division of Cancer Cause and Prevention, NCI, Frederick, MD and obtained from Dr. Sandra Ruscetti). In this study, we examined the ability of gemcitabine to inhibit replication of another retrovirus, murine leukemia virus in cell culture. Additionally, we examined the efficacy and toxicity of gemcitabine in vivo using LP-BM5 murine leukemia virus (LP-BM5 MuLV, a murine AIDS model). Gemcitabine decreased infectivity of MuLV in cell culture with an EC50 in the low nanomolar range with no detectable cytotoxicity. Similarly, gemcitabine significantly decreased disease progression in mice infected with LP-BM5.
Tags: bioactivity of anti-MuLVs 83A25; anti-MuLVs 83A25 of low endotoxin

Targeted Transduction Patterns in the Mouse Brain by Lentivirus Vectors Pseudotyped with VSV, Ebola, Mokola, LCMV, or MuLV Envelope Proteins
Watson DJ, et al. Mol Ther. 2002 Jul;6(1):25-34. doi: 10.1006/mthe.2002.0584. PMID: 12118993
MuLV-pseudotyped lentivirus efficiently transduced striatum and hippocampal dentate gyrus. In this study, CNS transduction patterns of lentiviral vectors pseudotyped with envelope glycoproteins from Ebola virus, murine leukemia virus (MuLV), lymphocytic choriomeningitis virus (LCMV), or the rabies-related Mokola virus were compared to a vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G). Mokola-, LCMV-, and VSV-G-pseudotyped vectors transduced similar populations, including striatum, thalamus, and white matter. Mokola-pseudotyped vectors were the most efficient of the three. In contrast, no transduction resulted from injection of Ebola-pseudotyped virus in the CNS.
Tags: anti-MuLVs (83A25) monoclonal antibody; clone 83A25 of MuLVs antibody

For more references about anti-MuLVs antibody (Clone: 83A25), please contact our scientific support team with message@sydlabs.com.