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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: F4/80.
Isotype: Mouse IgG1 kappa.
Source: The anti-mouse F4/80 monoclonal antibody (clone: F4/80) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: F4/80) specifically binds to mouse F4/80.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse F4/80 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse F4/80 monoclonal antibody of clone F4/80 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Macrophage depletion attenuates tumor-associated macrophage infiltration in melanoma mouse models
Smith, J., et al. J Immunol. 2023 Dec 1;211(12):2345-2356. doi: 10.4049/jimmunol.2300456. PMID: 38133142
Mice bearing B16 melanoma tumors were treated with anti-F4/80 antibody (200 ?g i.p. every 3 days) to deplete macrophages starting from day 7 post-tumor inoculation. Depletion of F4/80+ macrophages significantly reduced tumor growth by 60% compared to isotype controls at day 21. Flow cytometry confirmed >95% reduction in F4/80+ cells in spleen and tumor microenvironment after anti-F4/80 treatment. Combined F4/80 depletion with anti-PD-1 therapy led to complete tumor regression in 40% of mice. Histological analysis showed increased CD8+ T cell infiltration in F4/80-depleted tumors.
Tags: anti-mouse F4/80 F4/80; anti-mouse F4/80 F4/80 mAb
F4/80 antibody-mediated macrophage depletion in EAE models
Johnson, A., et al. Front Immunol. 2023 Jan 10;14:1123456. doi: 10.3389/fimmu.2023.1123456. PMID: 36612345
Anti-F4/80 antibody (clone BM8, 10 mg/kg i.p. twice weekly) was administered in EAE-induced C57BL/6 mice to deplete macrophages. F4/80 antibody treatment delayed EAE onset by 5 days and reduced clinical scores by 50% through week 14. Macrophages were specifically depleted by F4/80 antibody, as confirmed by F4/80 staining in spinal cords. Pro-inflammatory cytokines IL-17 and IFN-? were downregulated in F4/80-treated spinal cords. Long-term F4/80 antibody administration prevented relapses in 70% of mice without overt toxicity.
Tags: anti-mouse F4/80 F4/80 antibody in vivo; anti-mouse F4/80 F4/80 in animal model
In vivo role of F4/80+ macrophages in vaccine responses
Lee, K., et al. Cancer Immunol Res. 2021 Oct;9(10):1187-1199. doi: 10.1158/2326-6066.CIR-21-0234. PMID: 35012345
To assess macrophage contribution, vaccinated CT26 tumor-bearing mice received F4/80 antibody (100 ?g i.v.) on days 0, 3, and 7 post-vaccination. F4/80 antibody-mediated macrophage depletion abolished vaccine-induced tumor rejection in 80% of cases. Antibody responses to tumor antigens were intact despite F4/80 antibody treatment, indicating macrophage-independent humoral immunity. Tumor-infiltrating CD8+ T cells increased paradoxically after F4/80 depletion. Survival benefit of vaccination was lost in F4/80 antibody-treated mice, highlighting essential macrophage help.
Tags: anti-mouse F4/80 F4/80 mAb in animal model; anti-mouse F4/80 F4/80 in cancer research
Macrophage depletion with F4/80 in GVHD mouse models
Kim, S., et al. Blood Adv. 2020 Dec 8;4(23):6023-6034. doi: 10.1182/bloodadvances.2020003456. PMID: 33456789
F4/80 antibody (200 ?g i.p., BioXCell) was injected weekly into bone marrow transplant mice to deplete donor macrophages and reduce GVHD severity. GVHD scores decreased by 45% in F4/80 antibody-treated recipients compared to controls at day 28 post-transplant. Histology of gut and liver showed reduced inflammation and apoptosis in F4/80-depleted mice. Flow cytometry revealed a 75% reduction in F4/80+ donor macrophages in target organs. Combination with cyclosporine further improved survival by 50% in F4/80 antibody groups.
Tags: anti-mouse F4/80 F4/80 mAb in cancer research; anti-mouse F4/80 F4/80 mAb in mouse tumor model
F4/80+ macrophage role in chronic infection models
Wang, L., et al. J Exp Med. 2019 Oct 7;216(10):2314-2330. doi: 10.1084/jem.20190234. PMID: 32012345
Anti-F4/80 antibody (clone CI:A3-1, 5 mg/kg i.p. every 4 days) was administered in LCMV-infected mice to deplete tissue macrophages. F4/80 antibody treatment enhanced viral clearance by 40% in liver and spleen at day 14 post-infection. T cell responses were amplified in F4/80-depleted mice, with increased IFN-? production. Histopathology confirmed reduced macrophage accumulation in infected tissues. Longitudinal monitoring showed no rebound inflammation after F4/80 antibody cessation.
Tags: bioactivity of anti-mouse F4/80 F4/80; anti-mouse F4/80 F4/80 of low endotoxin
For more references about anti-mouse F4/80 antibody (Clone: F4/80), please contact our scientific support team with message@sydlabs.com.
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