Anti-Mouse F4 / 80 Monoclonal Antibodies

PA007640.m1: In Vivo Grade Recombinant Anti-mouse F4/80 Monoclonal Antibody (Clone: F4/80), Mouse IgG1 Kappa

Recombinant mouse IgG1 Monoclonal Antibody.
Clone: F4/80.
Isotype: Mouse IgG1 kappa.
Source: The anti-mouse F4/80 monoclonal antibody (clone: F4/80) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: F4/80) specifically binds to mouse F4/80.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse F4/80 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse F4/80 monoclonal antibody of clone F4/80 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse F4/80 antibody (Clone: F4/80):

1、Benzo (A) pyrene exposure alters alveolar epithelial and macrophage cells diversity and induces antioxidant responses in lungs.
Chauhan, P., et al. Toxicol Rep. 2024 Oct 18;13. doi: 10.1016/j.toxrep.2024.101777. PMID: 39506978
PE anti-mouse Podoplanin and Alexa Fluor 647 anti-mouse CD74 (CLIP), FITC anti-mouse CD11b, APC anti-mouse F4/80, APC rat IgG2bκ, FITC rat IgG2bκ, PE rat IgG2bκ, were purchased from Bio Legends (San Diego, CA, USA). ...After staining with anti-mouse CD11bFITC and the F4/80 pan macrophage marker, cells were analysed on a flow cytometer. ...The impacts of BaP on macrophage cells in the lungs were studied by staining the cells with the CD11b/F4/80 pan macrophage markers. ...A single-cell suspension from BaP-treated lungs was stained with anti-mouse CD11bFITC and F4/80 APC, followed by flow cytometric analysis. ...We have studied the macrophage response based on CD11b/F4/80 staining. Based on the surface expression analysis, three different types of macrophages were recognised, i.e., AM1, CD11b+, IM CD11b+F4/80+, and AM2, F4/80+.
Tags: anti-mouse F4/80 F4/80; anti-mouse F4/80 F4/80 mAb

2、Obesity does not diminish the efficacy of IL-6 signalling blockade in mice with collagen-induced arthritis.
Suzuki, M., et al. Clin Exp Rheumatol. 2017 Nov-Dec;35(6):893-898. PMID: 28421996
The mice fed the high-fat diet showed greater expression of macrophage marker F4/80, not only in subcutaneous fat but also in knee synovium and the calcaneal region, than did the mice fed a normal diet. ...Furthermore, macrophages isolated from mice on the high-fat diet tended to show higher expression of cyclooxygenase-2 following IL-6 stimulation than did macrophages from mice fed the normal diet. ...Moreover, mice fed the normal or high-fat diet were immunised with type II collagen, and were treated with anti-mouse IL-6 receptor antibody (MR16-1). ...The anti-arthritis effect of MR16-1 was not reduced in mice fed the high-fat diet compared to mice fed the normal diet (inhibition ratio: 87% vs. 62%). Furthermore, at the peak of arthritis, cyclooxygenase-2 expression in the calcaneal region of mice fed the high-fat diet was higher than that in the mice fed the normal diet. ...Obese rheumatoid arthritis patients often have higher disease activity and a poorer response to treatment than do non-obese patients.
Tags: anti-mouse F4/80 F4/80 antibody in vivo; anti-mouse F4/80 F4/80 in animal model

3、Colony stimulating factor 1 receptor blockade improves the efficacy of chemotherapy against human neuroblastoma in the absence of T lymphocytes.
Webb, M. W., et al. Int J Cancer. 2018 Sep 15;143(6):1483-1493. doi: 10.1002/ijc.31532. PMID: 29665011
In vivo, neuroblastomas formed by subcutaneous co-injection of human neuroblastoma cells and human monocytes into immunodeficient NOD/SCID mice had fewer human CD14+ and CD163+ cells and mouse F4/80+ cells after CSF-1R blockade. ...Monocytes and macrophages rely on colony stimulating factor-1 (CSF-1) and its receptor, CSF-1R, for recruitment and survival in the tumor microenvironment. ...Tumor-associated macrophages can promote growth of cancers. In neuroblastoma, tumor-associated macrophages have greater frequency in metastatic versus loco-regional tumors, and higher expression of genes associated with macrophages helps to predict poor prognosis in the 60% of high-risk patients who have MYCN-non-amplified disease. ...The contribution of cytotoxic T-lymphocytes to anti-neuroblastoma immune responses may be limited by low MHC class I expression and low exonic mutation frequency. ...Therefore, we modelled human neuroblastoma in T-cell deficient mice to examine whether depletion of monocytes/macrophages from the neuroblastoma microenvironment by blockade of CSF-1R can improve the response to chemotherapy.
Tags: anti-mouse F4/80 F4/80 mAb in animal model; anti-mouse F4/80 F4/80 in cancer research

4、Immunohistochemistry of Immune Cells and Cells Bound to in vivo Administered Antibodies in Liver, Lung, Pancreas, and Colon of B6/lpr Mice.
Adam, K., et al. Bio Protoc. 2022 Jul 20;12(14):e4468. doi: 10.21769/BioProtoc.4468. PMID: 35978576
Here, we describe the avidin-biotin conjugate (ABC) method used to stain T cells (CD4 and CD8), B cells (CD19), macrophages (F4/80), and cells bound by the in vivo administered rat anti-mouse antibodies for chromogenic immunohistochemistry (IHC). ...Using a biotinylated goat anti-rat antibody, we detected the localization of cells bound to the in vivo antibodies for PD-1 and CTLA-4. ...Additionally, detection and localization of in vivo antibodies can be used in mice models to infer their therapeutic efficacy, stability, and function. ...The last decade has seen the approval of several immune checkpoint blockade (ICB) antibodies in various cancers, which has improved patient survival. ...This method of detection could be used to evaluate ICB antibodies for toxicity in mice models.
Tags: anti-mouse F4/80 F4/80 mAb in cancer research; anti-mouse F4/80 F4/80 mAb in mouse tumor model

5、In Vivo Tumorigenesis, Osteolytic Sarcomas, and Tumorigenic Cell Lines from Transgenic Mice Expressing the Human T-Lymphotropic Virus Type 1 (HTLV-1) Tax Viral Oncogene.
Lanigan, L. G., et al. Am J Pathol. 2021 Feb;191(2):335-352. doi: 10.1016/j.ajpath.2020.10.014. PMID: 33181139
IHC analysis of the macrophage-specific antigen, F4/80, and HTLV-1 Tax were consistently positive in the histiocytic tumor cells. ...All other IHC staining performed on the tumors—CD3 for normal and neoplastic T cells, CD20 for normal and neoplastic B cells, pancytokeratin (AE1/AE3) for high- and low-molecular–weight keratins in epithelia, keratin 8 for nonsquamous/ductal epithelium, and S-100 for nonsquamous/ductal epithelium—were negative. ...As expected, F4/80 staining was largely restricted to the cell membrane, with a small amount of cytoplasmic staining (Figure 2, A and B). ...Round cells are more mature and have higher F4/80 expression, which increases with monocyte/macrophage maturation. ...The majority of F4/80+ cells had decreased cytoplasmic staining as they transitioned from differentiated round cells to less-differentiated, spindle-shaped cells, a key feature of HSs.
Tags: bioactivity of anti-mouse F4/80 F4/80; anti-mouse F4/80 F4/80 of low endotoxin

For more references about anti-mouse F4/80 antibody (Clone: F4/80) please contact our scientific support team with message@sydlabs.com.