Anti-Mouse Dendritic Cell Marker Monoclonal Antibodies

PA007602.r2b: In Vivo Grade Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (Clone: 33D1), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody.
Clone: 33D1.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse Dendritic Cell Marker monoclonal antibody (clone: 33D1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 33D1) specifically binds to mouse Dendritic Cell Marker.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Dendritic Cell Marker protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse Dendritic Cell Marker monoclonal antibody of clone 33D1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse Dendritic Cell Marker antibody (Clone: 33D1):

A 33D1+ Dendritic Cell/Autoreactive CD4+ T Cell Circuit Maintains IL-2-Dependent Regulatory T Cells in the Spleen
Sweigard JH, et al. Cell Rep. 2016 Oct 4;17(2):445-457. doi: 10.1016/j.celrep.2016.09.003. PMID: 27566821
Mice were treated with a single dose of 100μg of the indicated antibodies 48H prior to sacrifice. Tr cells compete for a limiting supply of Il-2. Rat IgG or Il-2 blocking antibody (S4B6-1) was administered to mice I.V. Data pooled from 4 independent experiments and pSTAT5 normalized to the untreated control Tr cells within each experiment. Il-2 activation (pSTAT5) in Tr cells from DC-depleted mice receiving 3x105 WT or β2M−/− 33D1+ CD11bint DCs.
Tags: anti-mouse Dendritic Cell Marker 33D1; anti-mouse Dendritic Cell Marker 33D1 mAb

Disruption of maternal immune balance maintained by innate DC subsets results in spontaneous pregnancy loss in mice
Nakayama T, et al. J Reprod Immunol. 2012 May;94(1):13-23. doi: 10.1016/j.jri.2011.12.004. PMID: 22325374
Similar miscarriages were also observed when pregnant mice were intraperitoneally (i.p.) injected twice with IL-12 on Gd 9.5 and 10.5. Moreover, prior inoculation of progesterone suppressed the enhanced serum IL-12 production in mice treated with 33D1 antibody. The Th1/Th2 balance seems to be regulated mainly by two distinct DC subsets, DEC-205(+) DCs having the capacity to establish Th1 polarization and 33D1(+) DCs to induce Th2 polarization. Dendritic cells (DCs) play an important role in providing an appropriate fetal/maternal balance between Th1 and Th2 during pregnancy. Using anti-33D1-specific monoclonal antibody, 33D1(+) DCs were successfully depleted from C57BL/6 mice.
Tags: anti-mouse Dendritic Cell Marker 33D1 in vivo; anti-mouse Dendritic Cell Marker 33D1 antibody in vivo

Effects of Dendritic Cell Subset Manipulation on Airway Allergy in a Mouse Model
Park JH, et al. Allergy Asthma Immunol Res. 2016 Jan;8(1):73-81. doi: 10.4168/aair.2016.8.1.73. PMID: 26855055
Th1 polarization can be achieved either by depletion of 33D1+ DCs with a 33D1-specific monoclonal antibody (mAb) or by activation of DEC-205+ DCs via intraperitoneal injection of α-galactosylceramide (α-GalCer). We studied the effect of 33D1+ DC depletion or DEC-205+ DC activation in vivo using an established mouse model of allergic rhinitis (AR). The allergic symptom scores were significantly decreased in 33D1+ DC-depleted or DEC-205+ DC-activated AR mice. The levels of OVA-specific IgG1, IgG2a, and IgE, and the number of NALF cells, but not BALF cells, were reduced in 33D1+ DC-depleted but not in DEC-205+ DC-activated AR mice. The manipulation of innate DC subsets may provide a new therapeutic strategy for controlling various allergic diseases by reducing histamine release from IgE-sensitized mast cells by driving the immune response towards Th1 dominancy via activation of DEC-205+ DCs in vivo.
Tags: anti-mouse Dendritic Cell Marker 33D1 in animal model; anti-mouse Dendritic Cell Marker 33D1 mAb in animal model

Antigen delivery to CD11c+CD8- dendritic cells induces protective immune responses against experimental melanoma in mice in vivo
Hartung E, et al. J Immunol. 2015 Jul 1;195(1):92-102. doi: 10.4049/jimmunol.1402977. PMID: 24829411
Ag delivery to select DC subpopulations via targeting Abs to DC inhibitory receptor 2 (DCIR2, clone 33D1) or to DEC205 was shown to direct Ags specifically to CD11c(+)CD8(-) or CD11c(+)CD8(+) DCs, respectively, in vivo. In this study, we demonstrate that Ag targeting to the CD11c(+)CD8(-) DC subpopulation in the presence of stimulating anti-CD40 Ab and TLR3 ligand polyinosinic-polycytidylic acid induces protective responses against rapidly growing tumor cells in naive animals under preventive and therapeutic treatment regimens in vivo. Dendritic cells (DCs) are central modulators of immune responses and, therefore, interesting target cells for the induction of antitumor immune responses. Unlike CD8+ DCs that express the cell surface protein CD205, CD8- DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies.
Tags: anti-mouse Dendritic Cell Marker 33D1 in cancer research; anti-mouse Dendritic Cell Marker 33D1 mAb in cancer research

Contact sensitizers decrease 33D1 expression on mature Langerhans cells
Aiba S, et al. J Invest Dermatol. 1999 May;112(5):814-8. doi: 10.1046/j.1523-1747.1999.00581.x. PMID: 10210782
In this study, the capacity of sensitizers, irritants and neutral chemicals to modulate the surface marker expression and morphology of pure mature murine Langerhans cells in vitro was examined. Contact with 4 sensitizers (2,4-dinitrobenzenesulfate, 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one, p-phenylenediamine, mercaptobenzo-thiazole) resulted in a rapid, specific, marked fall in 33D1 expression, a murine specific dendritic cell marker. Langerhans cells play a critical role in allergic contact hypersensitivity. In vivo, these cells capture xenobiotics that penetrate the skin and transport them through the lymphatic vessels into regional lymph nodes for presentation to T cells. During this migration step, Langerhans cells become mature, a process that is accompanied by a change in their surface marker expression.
Tags: function of anti-mouse Dendritic Cell Marker 33D1; anti-mouse Dendritic Cell Marker 33D1 mAb of low endotoxin

For more references about anti-mouse Dendritic Cell Marker antibody (Clone: 33D1), please contact our scientific support team with message@sydlabs.com.