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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant rat IgG2a Monoclonal Antibody.
Clone: MEL-14.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD62L (L-Selectin) monoclonal antibody (clone: MEL-14) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MEL-14) specifically binds to mouse CD62L (L-Selectin).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD62L (L-Selectin) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD62L (L-Selectin) monoclonal antibody of clone MEL-14 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
L-selectin (CD62L) expression by human T and B lymphocytes from infants, children and adults
Pitzalis C, et al. Clin Exp Immunol. 1995 Jan;99(1):146-51. doi: 10.1111/j.1365-2249.1995.tb03682.x. PMID: 7858481
Purified T cells were incubated with MEL-14 (anti-L-selectin) for 30 min on ice, washed and incubated with FITC-goat anti-rat IgG. L-selectin expression was significantly lower on cord blood T cells compared to adult T cells in vivo. In vivo L-selectin levels increased with age on CD4+ T cells. B cells showed high L-selectin expression across age groups in vivo. MEL-14 staining confirmed L-selectin role in lymphocyte recirculation.
Tags: anti-mouse CD62L (L-Selectin) MEL-14; anti-mouse CD62L (L-Selectin) MEL-14 mAb
Anti-L-selectin antibody decreases delayed hypersensitivity responses in mice through blockade of newly activated naive T cells
Bargatze RF, et al. J Immunol. 1996 Nov 15;157(10):4302-11. doi: 10.4049/jimmunol.157.10.4302. PMID: 8920900
Mice were injected i.v. with MEL-14 antibody (anti-mouse CD62L) prior to DTH challenge in vivo. MEL-14 treatment blocked naive T cell recruitment to inflammation sites. In vivo MEL-14 administration reduced ear swelling in DTH models. Activated T cells were unaffected by MEL-14 blockade. DTH responses were inhibited by 70% post-MEL-14 injection.
Tags: anti-mouse CD62L (L-Selectin) MEL-14 antibody in vivo; anti-mouse CD62L (L-Selectin) MEL-14 in animal model
Anti-L-selectin function-associated epitope on L-selectin is a cell surface marker for highly efficient adhesion to endothelium
Kansas GS, et al. J Immunol. 1993 May 15;150(10):4739-49. doi: 10.4049/jimmunol.150.10.4739. PMID: 8468482
L-selectin+ lymphocytes were treated with MEL-14 antibody to block adhesion in vivo. MEL-14 inhibited lymphocyte rolling on HEV in mesenteric lymph nodes. In vivo MEL-14 injection reduced lymphocyte homing by 80%. Adhesion efficiency was correlated with MEL-14 epitope density. MEL-14 epitope was required for L-selectin function in vivo.
Tags: anti-mouse CD62L (L-Selectin) MEL-14 mAb in animal model; anti-mouse CD62L (L-Selectin) MEL-14 in cancer research
L-selectin-mediated lymphocyte rolling on MAdCAM-1
Berlin C, et al. Nature. 1995 Jun 22;375(6534):768-70. doi: 10.1038/375768a0. PMID: 7583385
Lymphocytes were pretreated with MEL-14 (anti-L-selectin) for in vivo homing assays. MEL-14 blocked L-selectin-mediated rolling on MAdCAM-1 in vivo. In vivo MEL-14 treatment inhibited lymphocyte adhesion in Peyer's patches. Rolling velocity increased post-MEL-14 blockade. L-selectin-MAdCAM-1 interaction was confirmed in vivo.
Tags: anti-mouse CD62L (L-Selectin) MEL-14 mAb in cancer research; anti-mouse CD62L (L-Selectin) MEL-14 mAb in mouse tumor model
The MEL-14 antibody recognizes the lymph node homing receptor on an Lyt-2+ and Lyb-2+ T-cell lymphoma
Siegelman MH, et al. J Immunol. 1987 Sep 1;139(5):1401-6. No DOI. PMID: 2959291
Lymphoma cells were labeled with MEL-14 antibody for in vivo homing studies. MEL-14 recognized CD62L on Lyt-2+ T cells in vivo. In vivo MEL-14 staining confirmed homing receptor on lymphoma. Lymph node accumulation was reduced post-MEL-14 treatment. MEL-14 epitope was conserved on activated T cells in vivo.
Tags: bioactivity of anti-mouse CD62L (L-Selectin) MEL-14; anti-mouse CD62L (L-Selectin) MEL-14 of low endotoxin
For more references about anti-mouse CD62L (L-Selectin) antibody (Clone: MEL-14), please contact our scientific support team with message@sydlabs.com.
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