Anti-mouse CD62L (L-Selectin) (Clone: MEL-14) | Recombinant, in vivo grade

Catalog No.	Product Name	Size	List Price (US$)
PA007561.r2a	In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa	1 mg	150.00
PA007561.r2a	In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa	5 mg	350.00
PA007561.r2a	In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa	25 mg	900.00

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007561.r2a

In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa

1 mg

150.00

PA007561.r2a

In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa

5 mg

350.00

PA007561.r2a

In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa

25 mg

900.00

Description

PA007561.r2a: In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.
Clone: MEL-14.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD62L (L-Selectin) monoclonal antibody (clone: MEL-14) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MEL-14) specifically binds to mouse CD62L (L-Selectin).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD62L (L-Selectin) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse CD62L (L-Selectin) monoclonal antibody of clone MEL-14 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14)

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Brinkman, C. C., et al. Nat Commun. 2016 Jun 21;7:12021. doi: 10.1038/ncomms12021. PMID: 27323847

P values from Wilcoxon signed rank test for pre-injection to control IgG and anti-CD62L; Student’s t-test for control IgG versus anti-CD62L. In all, 1 pre-injection sample, 6 LN from control IgG treated animals and 9 LN from anti-CD62L-treated animals from 1 experiment, representative of 2. ...However, there were no differences in the ratio of Lta-/- to WT Treg between control and anti-CD62L-treated LN. ...After 18 h 100 µg anti-CD62L (MEL-14, BioXCell) or control Rat IgG2a (2A3, BioXCell) was injected i.v. After 18 h of antibody injection LN and spleen were collected and analysed by flow cytometry. ...For multiple comparisons, Tukey post tests were used when all possible comparisons were logically meaningful, and Bonferroni or Dunn’s post tests were used when only certain pairs of comparisons were experimentally or logically possible. ...Mouse LN and spleens were passed through 70 µm nylon mesh (Fisher Scientific) to produce single-cell suspensions and were enriched for CD4+ T cells using CD4+ negative selection (Stemcell Technologies).

(Tags: anti-mouse CD62L (L-Selectin) MEL-14; anti-mouse CD62L (L-Selectin) MEL-14 mAb)

Naïve T cells re-distribute to the lungs of selectin ligand deficient mice.

Harp, J. R., et al. PLoS One. 2010 Jun 4;5(6):e10973. doi: 10.1371/journal.pone.0010973. PMID: 20532047

Additionally, as shown later, if we treated recipient mice with blocking antibodies to L-selectin (anti-CD62L), we also observed reductions in naïve CD8 T cells in LNs, and increased T cells in lung and liver. ...Generally, when we inhibited naïve CD8 T cell entry to LN, via the HEVs using CD62L antibody blockade, genetic deficiency of selectin ligands, or inhibition of chemokine receptor signaling via G-coupled receptor signaling pathways, we observed more naïve CD8 T cells in the blood, and we also observed more naïve CD8 T cells in non-lymphoid organs. ...Moreover, we found that conditions that inhibit T cell trafficking to the LN, and increase the proportion of T cells in the blood, such as Ptx treatment and anti-CD62L blockade, also result in increased naïve CD8 T cell trafficking to non-lymphoid organs. ...The importance of elucidating mechanisms by which T cells, as well as other inflammatory cells, migrate into and out of the lung is underscored by the global health burden associated with lung diseases including COPD, asthma, and lung metastasis. ...For anti-CD62L experiments, 100 µg of MEL-14 (α-CD62L) blocking antibody (BioXcell, W. Lebanon, NH) was injected i.v. into WT recipient mice.

(Tags: anti-mouse CD62L (L-Selectin) MEL-14 antibody in vivo; anti-mouse CD62L (L-Selectin) MEL-14 in animal model)

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Anti-Mouse CD62L (L-Selectin) Monoclonal Antibodies

L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14

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PA007561.r2a: In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa

References of Anti-Mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14)