PA007487.r1: Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) Monoclonal Antibody (Clone: LOB12.3), Rat IgG1 Kappa
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: LOB12.3.
Isotype: Rat IgG1 Kappa.
Source: The anti-mouse CD137 (TNFRSF9 or 4-1BB) monoclonal antibody (clone: LOB12.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant LOB12.3 antibody specifically binds to mouse 4-1BB protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
PA007487.h1Fs: Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) Monoclonal Antibody (Clone: LOB12.3), Human IgG1 Fc Silent Kappa
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: LOB12.3.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD137 (TNFRSF9 or 4-1BB) monoclonal antibody (clone: LOB12.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant LOB12.3 antibody specifically binds to mouse 4-1BB protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The LOB12.3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
References of anti-mouse CD137 (TNFRSF9 or 4-1BB) antibody (LOB12.3)
FS222, a CD137/PD-L1 Tetravalent Bispecific Antibody, Exhibits Low Toxicity and Antitumor Activity in Colorectal Cancer Models.
Gaspar M, et al. Clin Cancer Res. 2020 Aug 1;26(15):4154-4167. doi: 10.1158/1078-0432.CCR-19-2958. PMID: 32273279
The results showed a clear difference between the two CD137 agonist antibodies tested, clone 3H3 induced a sustained increase in T cell infiltration, proliferation and activation in the liver and spleen, whereas clone Lob12.3 (anti-mouse CD137 antibody) did not (Fig. 6B). For cell binding assays cells were incubated with primary antibodies or mAb 2 followed by detection with an anti-human Fc-488 secondary antibody (Jackson Immunoresearch). Cells isolated from tissues and blood were stained for flow cytometry with fluorochrome conjugated antibodies including those against CD4, Ki67, FoxP3, CD69, CD3, CD8 (Thermo), CD45 (BD) and fixable live/dead dye (Thermo) in the presence of Fc block (Thermo) according to manufacturer’s recommendations. TIL analysis of day 21 CT26 tumors (n=5) treated with 1 mg/kg FS120 surrogate or controls Q2D starting on day 10 for 3 doses injected intraperitoneally by flow cytometry. Tumor growth was monitored by caliper measurements twice weekly.
Tags: anti-mouse CD137; anti-mouse CD137 LOB12.3
Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity.
Qi X, et al. Nat Commun. 2019 May 22;10(1):2141. doi: 10.1038/s41467-019-10088-1. PMID: 31105267
We observed similarly increased ALT levels in 3H3-treated naive mice (Fig. 1e, f), suggesting 3H3-induced liver toxicity is independent of tumor burden. Besides elevation of ALT in serum, we also found that immune cell infiltration in the liver of 3H3-treated mice (Fig. 1g). Our data suggested anti-4-1BB Ab-induced anti-tumor activity and liver toxicity could be separated in natural anti-4-1BB Abs. We, therefore, used LOB12.3 (anti-mouse CD137 antibody) and 3H3 Abs as tool molecules to investigate the underlying mechanism in order to gain insights for clinical development of agonistic anti-4-1BB Abs. Flow cytometry analysis showed increased CD8+ T cell infiltration in tumors treated with LOB12.3 compared to controls.
Tags: anti-mouse CD137 (4-1BB); anti-mouse CD137 (4-1BB) antibody
Unique potential of 4-1BB agonist antibody to promote durable regression of HPV+ tumors when combined with an E6/E7 peptide vaccine.
Bartkowiak T, et al. Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):E5290-9. doi: 10.1073/pnas.1514418112. PMID: 26369967
The agonistic anti-4-1BB antibody (clone Lob12.3) was administered intraperitoneally to enhance T cell responses in HPV+ tumor models. Mice were vaccinated with E6/E7 peptides on days 7 and 14 post-tumor implantation. Tumor growth was monitored by caliper measurements. Flow cytometry was used to assess CD8+ T cell infiltration using anti-CD8 (clone 53-6.7). Combination therapy led to durable tumor regression in 80% of treated mice.
Tags: anti-mouse CD137 antibody for animal model; anti-mouse CD137 antibody for flow cytometry
The EGR2 targets LAG-3 and 4-1BB describe and regulate dysfunctional antigen-specific CD8+ T cells in the tumor microenvironment.
Williams JB, et al. J Exp Med. 2017 Feb;214(2):441-460. doi: 10.1084/jem.20160485. PMID: 28077659
To confirm expression, tumor-infiltrating lymphocytes were stained with anti-4-1BB (clone LOB12.3) antibody for flow cytometry. Tumor-bearing mice were treated with anti-PD-1 therapy. Flow cytometry was performed using a BD LSRFortessa. CD8+ T cell dysfunction was assessed by LAG-3 and 4-1BB expression. Data were analyzed with FlowJo software.
Tags: anti-mouse CD137 for flow cytometry; anti-CD137 monoclonal antibody
Combined TIM-3 blockade and CD137 activation affords the long-term protection in a murine model of acute myeloid leukemia.
Zhou Q, et al. Blood. 2012 Nov 22;120(22):4291-301. doi: 10.1182/blood-2012-02-407098. PMID: 22976359
Anti-4-1BB (LOB12.3) antibody was injected intraperitoneally to activate CD137 signaling in AML models. Mice were challenged with C1498 leukemia cells. Flow cytometry analysis confirmed increased CD8+ T cell activation with LOB12.3 (anti-mouse CD137 antibody) treatment. Survival was monitored for 60 days post-treatment. Combination with anti-TIM-3 enhanced long-term protection.
Tags: LOB12.3 antibody for flow cytometry; LOB12.3 mAb for flow cytometry
For more references about anti-mouse CD137 (TNFRSF9 or 4-1BB) antibody (LOB12.3), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD137 (TNFRSF9 or 4-1BB) monoclonal antibodies:
Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) monoclonal antibody (Clone: 3H3)
Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) monoclonal antibody (Clone: LOB12.3)