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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 225.
Isotype: Mouse IgG1 Kappa.
Source:The anti-human EGFR monoclonal antibody (clone: 225) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 225) specifically binds to human EGFR.
Applications:ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human EGFR protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human EGFR monoclonal antibody of clone 225 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Near-infrared fluorescent molecular probes with cetuximab in the in vivo fluorescence imaging for epithelial ovarian cancer
Chen, Y., et al. J Ovarian Res. 2024 Nov 14;17(1):138. doi: 10.1186/s13048-024-01547-5. PMID: 39482752
Cetuximab (C225), a monoclonal antibody targeting EGFR, was conjugated with Cy7 for in vivo fluorescence imaging in a subcutaneous SKOV3-Luc xenograft model of epithelial ovarian cancer (EOC) with EGFR overexpression. NIRF imaging with 30 µg cetuximab-Cy7 enabled detection of tumor lesions, with ex vivo analysis showing fluorescence primarily in tumors and lungs compared to controls (PBS, free Cy7, and isotype IgG-Cy7).
Tags: anti-human EGFR 225; anti-human EGFR 225 mAb
Patient-derived xenograft models in cancer therapy: technologies and applications
Zhao, Y., et al. Signal Transduct Target Ther. 2023 Apr 11;8(1):147. doi: 10.1038/s41392-023-01419-2. PMID: 37094868
Patient-derived xenograft (PDX) models from drug-resistant patients identified MET mutations causing cetuximab resistance. These models, generated from therapy-resistant tumor cells, revealed HER2 as a therapeutic target in cetuximab-resistant colorectal cancer, highlighting PDX utility in studying cancer drug resistance mechanisms compared to standard mouse xenografts.
Tags: anti-human EGFR 225 antibody in vivo; anti-human EGFR 225 antibody in animal model
Inhibition of N-acetylglucosaminyltransferase V enhances the cetuximab-induced radiosensitivity of nasopharyngeal carcinoma cells likely through EGFR N-glycan alterations
Chen, Y., et al. Oncol Lett. 2017 Aug;14(2):1521-1528. doi: 10.3892/ol.2017.6319. PMID: 28535302
Cetuximab (C225), an EGFR inhibitor, enhanced radiosensitivity in nasopharyngeal carcinoma (NPC) cells when combined with N-acetylglucosaminyltransferase V (GnT-V) downregulation, which altered EGFR N-glycans. This combination reduced phospho-Akt and phospho-PI3K signaling and decelerated tumor growth in vivo, improving outcomes in CNE-2 cells compared to irradiation or cetuximab alone.
Tags: anti-human EGFR 225 in cancer research; anti-human EGFR 225 antibody in cancer research
Enhanced cytotoxic activity of cetuximab in EGFR-positive lung cancer by conjugating with gold nanoparticles
Kim, H., et al. Sci Rep. 2014 Dec 15;4:7808. doi: 10.1038/srep07808. PMID: 25502402
Cetuximab (C225), targeting EGFR-positive non-small cell lung cancer (NSCLC), was conjugated with 14 nm gold nanoparticles (AuNPs), enhancing cytotoxicity in A549 tumor-bearing mice both in vitro and in vivo. Compared to controls (saline, C225 alone, IgG-AuNPs), C225-AuNPs showed superior therapeutic potential for EGFR-positive NSCLC.
Tags: function of anti-human EGFR 225 antibody; bioactivity of anti-human EGFR 225 mAb
In vivo and ex vivo proofs of concept that cetuximab conjugated vitamin E TPGS micelles increases efficacy of delivered docetaxel against triple negative breast cancer
Yang, J., et al. Biomaterials. 2015 Aug;58:1-11. doi: 10.1016/j.biomaterials.2015.04.048. PMID: 26052086
Cetuximab-conjugated vitamin E TPGS micelles enhanced docetaxel delivery in a triple-negative breast cancer (TNBC) xenograft model using EGFR-overexpressing MDA-MB-231/Luc cells in SCID mice. In vivo biodistribution via IVIS® imaging showed improved tumor targeting, requiring a lower M−NP dosage to achieve tumor inhibition comparable to free drug.
Tags: anti-human EGFR antibody 225; clone 225 of anti-human EGFR mAb
For more references about anti-human CD3e antibody (225), please contact our scientific support team with message@sydlabs.com.
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