Anti-Human CD56 Monoclonal Antibodies for Flow Cytometry

PA007347.m2a: Non-conjugated Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG2a Kappa

Recombinant Mouse IgG2a Monoclonal Antibody. 
Clone: N901.
Isotype:  Mouse IgG2a kappa.
Source: The recombinant anti-human CD56 monoclonal antibody (clone: N901) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: N901) specifically binds to the human CD56 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007347.m1: Non-conjugated Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG1 Kappa

Recombinant Mouse IgG1 Monoclonal Antibody. 
Clone: N901.
Isotype:  Mouse IgG1 kappa.
Source: The recombinant anti-human CD56 monoclonal antibody (clone: N901) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: N901) specifically binds to the human CD56 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007347.h1Fs: Non-conjugated Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: N901.
Isotype:  Human IgG1 Fc Silent Kappa.
Source: The recombinant anti-human CD56 monoclonal antibody (clone: N901) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant N901 antibody specifically binds to the human CD56 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007348.m2a-b: Biotinylated Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG2a Kappa

Recombinant mouse IgG2a Monoclonal Antibody site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: N901.
Isotype: Mouse IgG2a kappa.
Source: The site-directed biotinylated recombinant anti-human CD56 monoclonal antibody (clone: N901) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant mouse monoclonal antibody (clone: N901)  specifically binds to the human CD56 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The N901 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background:

The recombinant N901 antibody binds to the human CD56 protein, a homophilic binding glycoprotein expressed on the surface of neurons, glia and skeletal muscle. CD56 is a marker of neural lineage commitment. CD56 expression is found in the hematopoietic system, natural killer cells, and other lymphoid cells, including gamma delta (γδ) Τ cells and activated CD8+ T cells, as well as on dendritic cells. CD56 has a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Syd Labs provides the following recombinant anti-human CD56 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
Biotin Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
FITC Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
APC Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
PE Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
Pacific Blue Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
PerCP Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
PE/Cy5 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
PE/Cy7 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
iFluor 488 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
iFluor 549 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
iFluor 594 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
iFluor 647 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
iFluor 700 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
APC/Cy7 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)
PerCP/Cy5.5 Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901)

References of anti-human CD56 antibody (N901):

Advanced Immune Cell Profiling by Multiparameter Flow Cytometry in Humanized Patient-Derived Tumor Mice
Bruss, C., et al. (2022). Cancers (Basel). 2022 Apr 28;14(9):2214. doi: 10.3390/cancers14092214. PMID: 35565343
The N901 antibody (anti-human CD56, Pe-conjugated) was used in multiparameter flow cytometry to characterize immune cells in humanized patient-derived tumor mice. Staining was performed on spleen single-cell suspensions and peripheral blood using FACSCanto-II or Navios cytometers, with DuraClone protocols, to assess CD56 expression alongside other markers like CD3, CD19, and CD45 for immune profiling.
Tags: activity of N901 antibody; function of N901 antibody

Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications
Blache, U., et al. (2021). Front Immunol. 2021 May 3;12:658314. doi: 10.3389/fimmu.2021.658314. PMID: 34012442
The N901 antibody (anti-human CD56) was included in antibody panels (TCR, RE ALB, CAR-T1, CAR-T2, NK1, NK2) for staining EDTA blood samples in CAR-T cell immune monitoring. Flow cytometry using a DxFLEX cytometer analyzed NK cell populations, with 100 µl whole blood stained for 15 minutes to assess CD56 expression in CAR-T therapy contexts.
Tags: N901 antibody; N901 antibody for cancer research

EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes
van Dongen, J. J. M., et al. (2012). Leukemia. 2012 Sep;26(9):1908-39. doi: 10.1038/leu.2012.120. PMID: 22552007
The N901 antibody (anti-human CD56) was part of the EuroFlow antibody panels for standardized flow cytometric immunophenotyping of leukocytes. Used with EuroFlow SOPs on FACSCanto-II cytometers and Infinicyt software, it facilitated consistent analysis of CD56 expression in normal, reactive, and malignant leukocytes, supporting WHO-based diagnosis of hematological malignancies.
Tags: N901 antibody for mouse tumor model; N901 antibody mouse dose range

Detection of the CD56+/CD45- immunophenotype by flow cytometry in neuroendocrine malignancies
Bryson, G. J., et al. (2002). J Clin Pathol. 2002 Jul;55(7):535-7. doi: 10.1136/jcp.55.7.535. PMID: 12101203
The N901 antibody (anti-human CD56, PE-conjugated) was used in a three-color flow cytometry assay (with anti-CD3-FITC and anti-CD45-PECY5) on a Beckman Coulter XL cytometer to identify the CD56+/CD45− immunophenotype in neuroendocrine malignancies, such as metastatic small cell carcinoma, confirming its utility in lymphoma evaluation.
Tags: N901 mAb for animal model; N901 mAb for cancer research

Aggressive natural killer cell leukemia/lymphoma with N901-positive surface phenotype: evidence for the existence of a third lineage in lymphoid cells
Imamura, N., et al. (1988). Acta Haematol. 1988;80(3):157-64. doi: 10.1159/000205617. PMID: 2461636
The N901 antibody (anti-human CD56, NKH-1) identified CD56 expression on leukemic cells with high NK activity in aggressive NK cell leukemia/lymphoma. Flow cytometry showed these cells lacked Leu 7 (HNK-1) and T/B-cell lineage markers, confirming a distinct third lymphoid lineage with no T beta, T gamma, or immunoglobulin gene rearrangement.
Tags: N901 mAb for mouse tumor model; N901 monoclonal antibody

For more references about anti-human CD56 antibody (N901), please contact our scientific support team with message@sydlabs.com.