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Conditions of optimal Ag85A Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Antigen 85A is a member of the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex have mycolyltransferase activity and catalyze the synthesis of the very rich glycolipid of the mycobacterial cell wall, the cord factor (trehalose 6,6'-dimycolate, TDM). The cord factor is vital for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A.
BP002108-PRO-1076: Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85A (Ag85A)
Source: Baculovirus-derived.
Recombinant Ag85A protein (43-338 a.a.) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa. Antigen 85A is fused to His tag at C-terminus and purified by conventional chromatographic techniques.
Purity: > 90% as determined by SDS-PAGE.
Formulation: The Ag85A solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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