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Conditions of optimal Actin Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Actin is a muscle protein localized in the I band of the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. Actin exists as a monomer in low salt concentrations, but filaments form rapidly as salt concentration rises, with the consequent hydrolysis of ATP. It occurs in globular (G-actin) and fibrous (F-actin) forms. Actin is found in all eukaryotic cells (except for nematode sperm). Actin is one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Its other functions include: cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.
BP000475-PRO-517: Actin Protein
Source: Rabbit Muscle-derived.
Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle.
Predicted molecular mass: 43,000 dalton.
Purity: > 98% by RP-HPLC and SDS-PAGE.
Formulation: Lyophilized from a 1 ug/ml solution containing 10 mM Tris/HCl buffer pH 8.0, 0.2 um CaCl2, 0.2 um ATP, 1 mM DTT and 0.5% (w/v) SDS.
Reconstitution: It is recommended to reconstitute the lyophilized Actin in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Applications: Immunoassays, Immunization.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Actin Antibodies:
Actin Polyclonal Antibody
Actin Monoclonal Antibody
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