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ACK1 (Phosphorylated) Antibody

ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; Non-receptor protein tyrosine kinase Ack; TNK2; kinase ACK1

Catalog No. Product Name Size List Price (US$) Quantity
PA000635-A0604 ACK1 (Phospho-Tyr284) Polyclonal Antibody 100 ug 350.00
PA000635-A0604 ACK1 (Phospho-Tyr284) Polyclonal Antibody 50 ug 250.00
Description

Introduction
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.

PA000635-A0604: ACK1 (Phospho-Tyr284) Polyclonal Antibody

Rabbit Polyclonal Antibody.
Specificity/Sensitivity: ACK1 (Phospho-Tyr284) antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
Applications: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:5000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Syd Labs provides the following ACK1 (Phosphorylated) Antibody :
ACK1 (Phosphorylated) Antibody

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