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Mouse GDF15 Protein

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

Catalog No. Product Name Size List Price (US$) Quantity
BP003920 Mouse GDF15 Protein with the N-His Tag 100UG 315.00
BP003920 Mouse GDF15 Protein with the N-His Tag 500UG 1050.00
BP005009 Mouse GDF15 Protein with the N-hFc Tag 100UG 315.00
BP005009 Mouse GDF15 Protein with the N-hFc Tag 500UG 1050.00
Description

BP003920: Mouse GDF15 Protein with the N-His Tag

Source: The recombinant Mouse GDF15 Protein, (amino acids Ser189-Ala303) fused with a His tag at the N-terminus, was produced in E.coli cells.
Molecular Weight:The recombinant Mouse GDF15 protein with the His tag at the N-terminus has a predicted MW of 13.78 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Mouse GDF15 protein with the N-His tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Mouse GFRAL protein with the N-His tag with the EC50 of 0.25 ug/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse GDF15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005009: Mouse GDF15 Protein with the N-hFc Tag

Source: The recombinant Mouse GDF15 Protein, (amino acids Ser189-Ala303) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Mouse GDF15 protein with the hFc tag at the N-terminus has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Mouse GFRAL protein with the N-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse GDF15 protein with the N-hFc tag with the EC50 of 3.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse GDF15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Mouse GDF15 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following GDF15 Protein :
Biotinylated Cynomolgus GDF15 Protein
Biotinylated Human GDF15 (H202D) Protein
Biotinylated Human GDF15 Protein
Biotinylated Mouse GDF15 Protein
Canine GDF15 Protein
Cynomolgus GDF15 Protein
Human GDF15 (H202D) Protein
Human GDF15 Protein
Mouse GDF15 Protein
Rat GDF15 Protein

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