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Human MICA Protein

FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250

Catalog No. Product Name Size List Price (US$) Quantity
BP003564 Human MICA Protein with the C-hFc Tag 100UG 315.00
BP003564 Human MICA Protein with the C-hFc Tag 500UG 1050.00
BP005512 Human MICA Protein with the C-His-Avi Tag 100UG 315.00
BP005512 Human MICA Protein with the C-His-Avi Tag 500UG 1050.00
Description

BP003564: Human MICA Protein with the C-hFc Tag

Source: The recombinant Human MICA Protein, (amino acids Glu24-Gln308) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human MICA protein with the hFc tag at the C-terminus has a predicted MW of 59.64 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human NKG2D protein with the C-hFc tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human MICA protein with the C-hFc tag with the EC50 of 3.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.36 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MICA Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005512: Human MICA Protein with the C-His-Avi Tag

Source: The recombinant Human MICA Protein, (amino acids Glu24-Gln308) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human MICA protein with the His-Avi tag at the C-terminus has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MICA protein with the C-His-Avi tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human NKG2D protein with the C-hFc tag with the EC50 of 0.81 ug/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human MICA Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human MICA Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following MICA Protein :
Biotinylated Human MICA Protein
Cynomolgus MICA Protein
Human MICA alpha 3 Protein
Human MICA Protein

Syd Labs provides the following MICA ELISA Kit :
Human MICA ELISA Kit

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