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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal recombinant Human GPC3/Glypican 3 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005337: Human GPC3/Glypican 3 Protein with the C-His Tag
Source: The recombinant Human GPC3/Glypican 3 Protein, (amino acids Gln25-His559) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human GPC3/Glypican 3 protein with the His tag at the C-terminus has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human GPC3 protein with the C-His tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-GPC3 Antibody protein with the C-hFc tag with the EC50 of 4.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human GPC3/Glypican 3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Human GPC3/Glypican 3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following GPC3 Protein :
Biotinylated Human GPC3 Protein
Cynomolgus GPC3 Protein
Human GPC3 (438-554) Protein-VLP
Human GPC3 Protein
Human GPC3 Protein-VLP
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