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Human Fc gamma RIIIA Protein

CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

Catalog No. Product Name Size List Price (US$) Quantity
BP005405 Human Fc gamma RIIIA/CD16a (V176) Protein with the C-His-Avi Tag 100UG 240.00
BP005405 Human Fc gamma RIIIA/CD16a (V176) Protein with the C-His-Avi Tag 500UG 800.00
Description

BP005405: Human Fc gamma RIIIA/CD16a (V176) Protein with the C-His-Avi Tag

Source: The recombinant Human Fc gamma RIIIA/CD16a (V176) Protein, (amino acids Gly17-Gln208(V176)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Fc gamma RIIIA/CD16a (V176) protein with the His-Avi tag at the C-terminus has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Fc gamma RIIIA (V176) protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-Fc gamma RIIIA Antibody protein with the C-hFc tag with the EC50 of 23.3 ng/ml determined by ELISA (QC test). The affinity constant of 67.3 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Fc gamma RIIIA/CD16a (V176) Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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