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Conditions of optimal recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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BP004993: Biotinylated Human Nectin-1/PVRL1/CD111 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein, (amino acids Gln31-Thr334) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human Nectin-1/PVRL1/CD111 protein with the His-Avi tag at the C-terminus has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Nectin-3 protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human Nectin-1 protein with the C-His tag with the EC50 of 0.52 ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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