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Conditions of optimal recombinant Biotinylated Human GDF15 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP004144: Biotinylated Human GDF15 Protein with the N-His-Avi Tag
Source: The recombinant Biotinylated Human GDF15 Protein, (amino acids Ala197-Ile308) fused with a His-Avi tag at the N-terminus, was produced in E.coli cells.
Molecular Weight:The recombinant Biotinylated Human GDF15 protein with the His-Avi tag at the N-terminus has a predicted MW of 15.19 kDa same as Tris-Bis PAGE result.
Activity: Immobilized recombinant Human GFRAL protein with the N-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human GDF15 protein with the N-His tag with the EC50 of 24 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human GDF15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005010: Biotinylated Human GDF15 Protein with the N-hFc Tag
Source: The recombinant Biotinylated Human GDF15 Protein, (amino acids Ala197-Ile308) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human GDF15 protein with the hFc tag at the N-terminus has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human GFRAL protein with the N-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human GDF15 protein with the N-hFc tag with the EC50 of 7.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human GDF15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Biotinylated Human GDF15 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following GDF15 Protein :
Biotinylated Cynomolgus GDF15 Protein
Biotinylated Human GDF15 (H202D) Protein
Biotinylated Human GDF15 Protein
Biotinylated Mouse GDF15 Protein
Canine GDF15 Protein
Cynomolgus GDF15 Protein
Human GDF15 (H202D) Protein
Human GDF15 Protein
Mouse GDF15 Protein
Rat GDF15 Protein
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