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Biotinylated Human FGL1 Protein

FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand

Catalog No. Product Name Size List Price (US$) Quantity
BP003549 Biotinylated Human FGL1 Protein with the N-hFc Tag 100UG 525.00
BP003549 Biotinylated Human FGL1 Protein with the N-hFc Tag 500UG 1750.00
BP005450 Biotinylated Human FGL1 Protein with the N-His-Avi Tag 100UG 525.00
BP005450 Biotinylated Human FGL1 Protein with the N-His-Avi Tag 500UG 1750.00
Description

BP003549: Biotinylated Human FGL1 Protein with the N-hFc Tag

Source: The recombinant Biotinylated Human FGL1 Protein, (amino acids Asp64-Asn305) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human FGL1 protein with the hFc tag at the N-terminus has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human LAG3 protein with the N-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human FGL1 protein with the N-hFc tag with the EC50 of 4.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human FGL1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005450: Biotinylated Human FGL1 Protein with the N-His-Avi Tag

Source: The recombinant Biotinylated Human FGL1 Protein, (amino acids Asp64-Asn305) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human FGL1 protein with the His-Avi tag at the N-terminus has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human FGL1 protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human LAG3 protein with the N-hFc tag with the EC50 of 0.16 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human FGL1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Biotinylated Human FGL1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following FGL1 Protein :
Biotinylated Human FGL1 Protein
Cynomolgus FGL1 Protein
Human FGL1 Protein

Syd Labs provides the following FGL2 Protein :
Biotinylated Cynomolgus FGL2 Protein
Biotinylated Human FGL2 Protein
Biotinylated Mouse FGL2 Protein
Biotinylated Rat FGL2 Protein
Cynomolgus FGL2 Protein
Human FGL2 Protein
Mouse FGL2 Protein

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