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Conditions of optimal recombinant Biotinylated Human E-Selectin/CD62E Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005666: Biotinylated Human E-Selectin/CD62E Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human E-Selectin/CD62E Protein, (amino acids Trp22-Pro556) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human E-Selectin/CD62E protein with the His-Avi tag at the C-terminus has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human E-selectin protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant Anti-E-Selectin Antibody protein with the C-hFc tag with the EC50 of 3.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human E-Selectin/CD62E Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Biotinylated Human E-Selectin/CD62E Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following E-selectin Protein :
Biotinylated Human E-Selectin Protein
Cynomolgus E-selectin Protein
E-selectin-Fc Protein
Human E-Selectin Protein
Mouse E-Selectin Protein
Syd Labs provides the following E-Selectin ELISA Kit :
E-Selectin ELISA Kit
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