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Conditions of optimal recombinant Biotinylated Human DNAM-1/CD226 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005049: Biotinylated Human DNAM-1/CD226 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human DNAM-1/CD226 Protein, (amino acids Glu19-Asn247) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human DNAM-1/CD226 protein with the His-Avi tag at the C-terminus has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human DNAM-1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate(5 ug/ml). Dose response curve for the recombinant Human CD155 protein with the C-mFc tag with the EC50 of 11.0 ng/ml determined by ELISA (QC Test). The affinity constant of 264 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human DNAM-1/CD226 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Biotinylated Human DNAM-1/CD226 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following DNAM-1 Protein :
Biotinylated Human DNAM-1 Protein
Cynomolgus DNAM-1 Protein
Human DNAM-1 Protein
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