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VEGFR1 ELISA Kit

FLT1 ELISA Kit
Catalog No. Product Name Size List Price (US$) Quantity
EK000087-EK0543-1 Human VEGFR1 ELISA Kit 1 plate, 96T/plate 444.00
EK000087-EK0543-4 Human VEGFR1 ELISA Kit 4 plates, 96T/plate 1510.00
Description

EK000087-EK0543: Human VEGFR1 ELISA Kit

Range 31.2pg/ml-2000pg/ml
Sensitivity < 4pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human VEGFR1 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

VEGFR1, also known as FMS-related tyrosine kinase 1(FLT1). Oncogene FLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. FLT is mapped to 13q12. VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human VEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa

Principle

The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.

Reference

1. Imbert, A.; Rosnet, O.; Marchetto, S.; Ollendorff, V.; Birnbaum, D.; Pebusque, M.-J. : Characterization of a yeast artificial chromosome from human chromosome band 13q12 containing the FLT1 and FLT3 receptor-type tyrosine kinase genes. Cytogenet. Cell Genet. 67: 175-177, 1994.
2. Niida, S.; Kondo, T.; Hiratsuka, S.; Hayashi, S.-I.; Amizuka, N.; Noda, T.; Ikeda, K.; Shibuya, M. : VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc. Nat. Acad. Sci. 102: 14016-14021, 2005.

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