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VEGF-C ELISA Kit

Catalog No. Product Name Size List Price (US$) Quantity
EK000098-EK0588-1 Human VEGF-C ELISA Kit 1 plate, 96T/plate 516.00
EK000098-EK0588-4 Human VEGF-C ELISA Kit 4 plates, 96T/plate 1754.00
Description

EK000098-EK0588: Human VEGF-C ELISA Kit

Range 31.2pg/ml-2000pg/ml
(human sera, body fluids )
Sensitivity < 2pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human VEGF-C in sera, serum, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.

Principle

The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.

Reference

1. Joukov, V.; Pajusola, K.; Kaipainen, A.; Chilov, D.; Lantinen, I.; Kukk, E.; Saksela, O.; Kalkkinen, N.; Alitalo, K. : A novel vascular endothelial growth factor, VEGF-C, is a ligand for the Flt4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases. EMBO J. 15: 290-298, 1996.
2. Lee, J.; Gray, A.; Yuan, J.; Luoh, S.-M.; Avraham, H.; Wood, W. I. : Vascular endothelial growth factor-related protein: a ligand and specific activator of the tyrosine kinase receptor Flt4. Proc. Nat. Acad. Sci. 93: 1988-1992, 1996.

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