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Home > Services > Antibody Services > Custom Monoclonal Antibody Services > Monoclonal Antibody Immunoconjugation Service

Monoclonal Antibody Immunoconjugation Service

Catalog No. Product Name Size List Price (US$) Quantity
BS039A Pilot Study Phase I: Generation of antibody immunoconjugates 1 package Request
BS039B Pilot Study Phase II: In vitro Bioassay 1 package Request
BS039C Medium-size Production Phase I: Generation of antibody immunoconjugates 1 package Request
BS039D Medium-size Production Phase II: In vitro Bioassay 1 package Request
BS039E Medium-size Production Phase III: In vivo animal study 1 package Request
Description
1. Pilot Study

Material required
3~5 mg purified monoclonal antibody needed

Phase I Generation of antibody immunoconjugates
? Exchange the antibody buffer to the conjugation buffer
? Activation of the antibody
? Conjugation of the antibody to TWO different drugs at TWO different molar ratio
? Purification of the immunoconjugates
? Filter-sterilization

Phase II In vitro Bioassay
? Seeding the cells in 96-well plate, up to THREE different cell lines
? Adding the immunoconjugates at various concentrations.
? Performing MTT assay on day 5.
? Repeat the bioassay twice

2. Medium-size Production

Material required
5~10 mg purified monoclonal antibody needed

Phase I Generation of antibody immunoconjugates
? Exchange the antibody buffer to the conjugation buffer
? Activation of the antibody
? Conjugation of the antibody to ONE drug at TWO different molar ratio
? Purification of the immunoconjugates
? Filter-sterilization

Phase II In vitro Bioassay
? Seeding the cells in 96-well plate, up to THREE different cell lines
? Adding the immunoconjugates at various conc.
? Performing MTT assay on day 5.
? Repeat the bioassay once
? Sending out 2 mg immunoconjugate.

Phase III In vivo animal study
? Innoculate 30 nude mice with the right number of tumor cells
? Once the tumors reach 10 mm, 5 mice per group will be injected with various amount of immunoconjugate, naked antibody, unconjugated drug and buffer
? Tumor size will be measured every 3 or 4 days
? At the end of the test, the tumors will be removed and weighed individually.
? Repeat the animal study twice.
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