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Conditions of optimal SEMA5A Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Introduction
Semaphorin 5A (Sema5A, previously called SemaF) is a member of the semaphorin family of axon guidance molecules . Sema5A is a 140 kDa protein. Class 5 semaphorins are type I transmembrane glycoproteins with an N- terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains . Sema5A is expressed in neuroepithelial cells surrounding retinal axons, oligodendrocytes, the base of limb buds, the mesoderm surrounding cranial vessels , and the cardiac atrial septum and endocardial cushions, Human Sema5A cDNA encodes a signal sequence, a extracellular domain (ECD), a transmembrane sequence and an cytoplasmic portion. Sema5A mutations have been implicated in the genetic syndrome,cri-du-chat,while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson. The expression of Sema5A may upregulated in metastatic cancer cells and downregulated in autism.
BP001095-C499: Recombinant Human Semaphorin-5A (SEMA5A) Protein
Source: HEK 293-derived.
Recombinant Human Semaphorin-5A (SEMA5A) Protein is a secreted protein with sequence (Glu23-Thr765) of Human SEMA5A fused with a polyhistidine tag at the C-terminus.
Predicted molecular mass: 140 kDa
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, 0.1 mM EDTA, 0.05% Tween 20, pH 7.2.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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