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EK000129-EK0882: Rat KIM1 ELISA Kit
Range 31.2pg/ml-2000pg/ml
Sensitivity < 2pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of rat KIM1 in sera, serum, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
TIM-1 also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant Rat KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
Principle
KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat KIM1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The Rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat KIM1 amount of sample captured in plate.
Reference
1. ^ Feigelstock D, Thompson P, Mattoo P, Zhang Y, Kaplan GG (Aug 1998). "The Rat homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor". J Virol 72 (8): 6621?.
2. ^ McIntire JJ, Umetsu SE, Akbari O, Potter M, Kuchroo VK, Barsh GS, Freeman GJ, Umetsu DT, DeKruyff RH (Nov 2001). "Identification of Tapr (an airway hyperreactivity regulatory locus) and the linked Tim gene family". Nat Immunol 2 (12): 1109?6. doi:10.1038/ni739..
3. ^ "Entrez Gene: HAVCR1 hepatitis A virus cellular receptor 1".
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=26762
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