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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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The cheapest Blood Direct PCR Kits and PCR Master Mixes in the market. Free samples of Blood Direct PCR Kits and PCR Master Mixes may be available.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Blood Direct PCR Kit can be used for both easy and rapid genomic DNA isolation and preparation from fresh or anti-coagulated blood coupled with PCR.
Rapid genomic DNA extraction:
Step 1:
Blood (fresh or anti-coagulated): Add 5-10 ul blood into a 0.5 ml microcentrifuge tube containing 200 ul solution C, vortex briefly, and incubate at room temperature for 3 minutes. Centrifuge at 6000 rpm for 1 minute, and remove supernantant completely. Add 100 ul solution A to the tube.
Step 2:
Place the tube in a thermocycler and heat at 95°C for 5 min. Cool the tube to room temperature. Add 10 ul solution B and vortex briefly. Extracted genomic DNA is now ready for use as PCR temperate or for longer term storage at -20°C.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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