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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal PCNA antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
PA000079-MA1083: PCNA Monoclonal Antibody
Mouse Monoclonal Antibody.
Specificity/Sensitivity: Human, mouse, rat. No cross reactivity with other proteins.
Applications: Western blot: At 2ug/ml with the appropriate system to detect PCNA in cells and tissues. Immunohistochemistry(P): At 0.4-1 ug/ml to detect PCNA in formalin fixed and paraffin embedded tissues. Boiling the sections may improve the staining. Immunohistochemistry(F): At 0.4-1 ug/ml to detect PCNA in formalin or acetone fixed tissues.
Reactivity: Human, Mouse, Rat.
Immunogen: Protein A fusion protein
Purification: Purified by the goat anti-mouse IgG affinity chromatography.
Form of Antibody: Lyophilized from 1.2% sodium acetate, with 2 mg BSA and 0.01 mg NaN3 as preservative.
Clone: IML-83.
Isotype: Mouse IgG2a.
Storage/Stability: Stable for 1 year at 0 °C and 3 months at 4 °C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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