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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal p65 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Nuclear Factor kappa B, a transcription factor complex, is ubiquitously expressed in almost all animal cells and involved in cellular responses to various stimuli, such as stress, cytokines, free radicals, bacterial or viral antigens, and etc. Specially, NF-kB plays a key role in the regulation of immune responses. It is kept in cytoplasm by specific inhibitors. Upon degradation of inhibitors after stimulation, NF-kB translocate into nucleus and activate downstream gene expression. NF-kB is composed of NF-kB1 or NF-kB2 bound to either REL, RELA or RELB. The most abundant heterdimeric NF-kB is NF-kB1 complexed with RELA.
BP001020-Z65: Recombinant Human transcription factor p65 protein
Source: Baculovirus, SF9 insect cells-derived.
Predicted molecular mass: 63.6 kDa.
Bioactivity test: 1 unit equals 1 nanogram of purified protein. 10 unit is sufficient for a gel mobility shift assay in a 20 ul reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity: > 90%, by SDS-PAGE.
Endotoxin Level: < 1.0 EU per 1 ug of the protein by the LAL method.
Formulation: 25 mM HEPES (pH 7.5), 20% glycerol, 150 mM NaCl, 0.1 mM TCEP 2 mM EDTA, carrier-free.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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