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Conditions of optimal recombinant Mouse VEGF R3/FLT4 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP003315: Mouse VEGF R3/FLT4 Protein with the C-hFc Tag
Source: The recombinant Mouse VEGF R3/FLT4 Protein, (amino acids Tyr25-Glu775) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Mouse VEGF R3/FLT4 protein with the hFc tag at the C-terminus has a predicted MW of 111.69 kDa. Due to glycosylation, the protein migrates to 115-160 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Mouse VEGF R3 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGF-C protein with the C-His tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse VEGF R3/FLT4 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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