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Conditions of optimal recombinant Mouse SPARC Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP004881: Mouse SPARC Protein with the C-His Tag
Source: The recombinant Mouse SPARC Protein, (amino acids Ala18-Ile302) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Mouse SPARC protein with the His tag at the C-terminus has a predicted MW of 33.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse SPARC Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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