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EK000147-EK0825: Mouse LOX-1 ELISA Kit
Range 31.2pg/ml-2000pg/ml;
Sensitivity < 1pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of mouse LOX-1 in serum, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. It belongs to the C-type lectin superfamily. The LOX1 gene is mapped to to 12p13-p12. The protein has got the amino acids of 363. It can be detected in various tissues, highly expressed in placenta. This protein may be involved in the regulation of Fas-induced apoptosis and play a role as a scavenger receptor. The standards of this kit are recombinant mouse LOX-1 (R60-I363), with molecular weight of 36.3kDa.
Principle
The mouse LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse LOX-1 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LOX-1 amount of sample captured in plate.
Reference
1. Li X, Bouzyk MM, Wang X (Nov 1998). "Assignment of the human oxidized low-density lipoprotein receptor gene (OLR1) to chromosome 12p13.1?p12.3, and identification of a polymorphic CA-repeat marker in the OLR1 gene". Cytogenet Cell Genet 82 (1-2): 34?. doi:10.1159/000015059. PMID 9763655.
2. Honjo, M., Sawamura, T., Hinagata, J., Nakamura, K., Sanada, N., Tanihara, H., Honda, Y., Kiryu, J. Expression of LOX-1, an oxidized low-density lipoprotein receptor, in choroidal neovascularization. Arch. Ophthal. 122: 1873-1876, 2004.
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