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EK000126-EK0780: Mouse IL-7 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 1pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of mouse IL-7 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
Interleukin 7 (IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17 (T(H)17) cells in experimental autoimmune encephalomyelitis (EAE).2
Principle
IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-7 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-7 amount of sample captured in plate.
Reference
1.Sutherland, G. R., Baker, E., Fernandez, K. E. W., Callen, D. F., Goodwin, R. G., Lupton, S., Namen, A. E., Shannon, M. F., Vadas, M. A. The gene for human interleukin 7 (IL7) is at 8q12-13. Hum. Genet. 82: 371-372, 1989.
2.Liu, X., Leung, S., Wang, C., Tan, Z., Wang, J., Guo, T. B., Fang, L., Zhao, Y., Wan, B., Qin, X., Lu, L., Li, R., Pan, H., Song, M., Liu, A., Hong, J., Lu, H., Zhang, J. Z. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. Nature Med. 16: 191-197, 2010. [PubMed: 20062065
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