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EK000062-EK0408: Mouse IL-5 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 2 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of mouse IL-5 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Pereira, E.; Goldblatt, J.; Rye, P.; Sanderson, C.; Le Souef, P. Mutation analysis of interleukin-5 in an asthmatic cohort. Hum. Mutat. 11: 51-54, 1998.
2. Campbell, H. D.; Tucker, W. Q. J.; Hort, Y.; Martinson, M. E.; Mayo, G.; Clutterbuck, E. J.; Sanderson, C. J.; Young, I. G. Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin-5). Proc. Nat. Acad. Sci. 84: 6629-6633, 1987.
3. Le Beau, M. M.; Lemons, R. S.; Espinosa, R., III; Larson, R. A.; Arai, N.; Rowley, J. D. Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q). Blood 73: 647-650, 1989.
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