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EK000125-EK0866: Mouse IL-23 ELISA Kit
Range 31.2pg/ml-2000pg/ml
Sensitivity < 2pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of mouse IL-23 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).
Principle
IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-23 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection monoclonal antibodies (clone No. 35928.11) were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate.
Reference
1. Oppmann B, Lesley R, Blom B, Timans JC, Xu Y, Hunte B, Vega F, Yu N, Wang J, Singh K, Zonin F, Vaisberg E, Churakova T, Liu M, Gorman D, Wagner J, Zurawski S, Liu Y, Abrams JS, Moore KW, Rennick D, de Waal-Malefyt R, Hannum C, Bazan JF, Kastelein RA (Jan 2001). "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12". Immunity 13 (5): 715?5.
2. ^ a b "Entrez Gene: IL23A interleukin 23, alpha subunit p19".
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51561.
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