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Conditions of optimal recombinant Mouse CD73/NT5E Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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BP005382: Mouse CD73/NT5E Protein with the C-His Tag
Source: The recombinant Mouse CD73/NT5E Protein, (amino acids Trp29-Lys549) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Mouse CD73/NT5E protein with the His tag at the C-terminus has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Mouse CD73 protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD73 Antibody protein with the C-hFc tag with the EC50 of 12.7 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse CD73/NT5E Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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