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Conditions of optimal IL-1β Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
IL-1 is a name that designates two proteins, IL-1α and IL-1β, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show apprximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1β, designated interleukin 1β-converting enzyme (ICE), has been described. Mature human and mouse IL-1? share approximately 75% amino acid sequence identity and human IL-1β has been found to be active on murine cell lines.
BP000132-C042: Recombinant Mouse Interleukin-1β (IL-1β) Protein
Source: E. coli-derived.
Recombinant Mouse Interleukin-1β (IL-1β) Protein is a non-glycosylated polypeptide chain containing 152 amino acids with a molecular mass of 17,400 Daltons.
N-terminal sequence analysis: Val-Pro-Ile-Arg-Gln.
Predicted molecular mass: 17400 Dalton.
The ED50 as determined by the dose-dependant stimulation of mouse D10S cells was found to be less than 0.01 ng/ml, corresponding to a Specific Activity of 1.0 x 108 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 um filtered solution of 50 mM Tris-HCl, 50 mM NaCl, pH 8.0.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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