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EK000099-EK0642: Human TSP2 ELISA Kit
Range 156pg/ml-10ng/ml
Sensitivity < 5pg/ml
Specificity: no detectable cross-reactivity with any other cytokine
Application: for quantitative detection of human TSP2 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Thrombospondin-2(TSP-2) is a protein that in humans is encoded by the THBS2 gene.1 And this gene is mapped to 6q27.2 The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.3 The standard product used in this kit is isolated from human plasma with the molecular mass of 129KDa.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Hirose Y, Chiba K, Karasugi T, Nakajima M, Kawaguchi Y, Mikami Y, Furuichi T, Mio F, Miyake A, Miyamoto T, Ozaki K, Takahashi A, Mizuta H, Kubo T, Kimura T, Tanaka T, Toyama Y, Ikegawa S (May 2008). "A functional polymorphism in THBS2 that affects alternative splicing and MMP binding is associated with lumbar-disc herniation". Am J Hum Genet 82 (5): 1122?9.
2. LaBell, T. L.; McGookey Milewicz, D. J.; Disteche, C. M.; Byers, P. H. : Thrombospondin II: partial cDNA sequence, chromosome location, and expression of a second member of the thrombospondin gene family in humans. Genomics 12: 421-429, 1992.
3. Bein, K; Simons M (Oct. 2000). "Thrombospondin type 1 repeats interact with matrix metalloproteinase 2. Regulation of metalloproteinase activity". J. Biol. Chem. (UNITED STATES) 275 (41): 32167?73.
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