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Human TREM2 Protein

PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c

Catalog No. Product Name Size List Price (US$) Quantity
BP004791 Human TREM2 Protein with the C-His Tag 100 ug 315.00
BP004791 Human TREM2 Protein with the C-His Tag 500 ug 1050.00
BP004779 Human TREM2 Protein with the C-hFc Tag 100 ug 203.00
BP004779 Human TREM2 Protein with the C-hFc Tag 500 ug 675.00
Description

BP004791: Human TREM2 Protein with the C-His Tag

Source: The recombinant Human TREM2 Protein, (amino acids His19-Ser174) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human TREM2 protein with the His tag at the C-terminus has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human TREM2 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-TREM2 Antibody protein with the C-hFc tag with the EC50 of 2.6 ng/ml determined by ELISA (QC Test). The affinity constant of 20.24 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human TREM2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP004779: Human TREM2 Protein with the C-hFc Tag

Source: The recombinant Human TREM2 Protein, (amino acids His19-Ser174) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human TREM2 protein with the hFc tag at the C-terminus has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human TREM2 protein with the C-hFc tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-TREM2 Antibody protein with the C-hFc tag with the EC50 of 46.9 ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human TREM2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human TREM2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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