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Conditions of optimal recombinant Human TNFSF12/TWEAK Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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BP003809: Human TNFSF12/TWEAK Protein with the C-hFc Tag
Source: The recombinant Human TNFSF12/TWEAK Protein, (amino acids Ser43-His249) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human TNFSF12/TWEAK protein with the hFc tag at the C-terminus has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human TNFSF12 protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-TNFSF12 Antibody protein with the C-hFc tag with the EC50 of 0.14 ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human TNFSF12/TWEAK Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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