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Conditions of optimal recombinant Human SPARC Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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BP004880: Human SPARC Protein with the C-His Tag
Source: The recombinant Human SPARC Protein, (amino acids Ala18-Ile303) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human SPARC protein with the His tag at the C-terminus has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human SPARC protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-SPARC Antibody protein with the C-hFc tag with the EC50 of 46.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human SPARC Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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