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Human RNF43 Protein

E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43

Catalog No. Product Name Size List Price (US$) Quantity
BP003229 Human RNF43 Protein with the C-His Tag 100 ug 315.00
BP003229 Human RNF43 Protein with the C-His Tag 500 ug 1050.00
BP004534 Human RNF43 Protein with the C-hFc Tag 100 ug 270.00
BP004534 Human RNF43 Protein with the C-hFc Tag 500 ug 900.00
Description

BP003229: Human RNF43 Protein with the C-His Tag

Source: The recombinant Human RNF43 Protein, (amino acids Gly24-Tyr197) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human RNF43 protein with the His tag at the C-terminus has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.
Activity: The affinity constant of 0.255 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human RNF43 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP004534: Human RNF43 Protein with the C-hFc Tag

Source: The recombinant Human RNF43 Protein, (amino acids Gly24-Tyr197) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human RNF43 protein with the hFc tag at the C-terminus has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human R spondin 1 protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human RNF34 protein with the C-hFc tag with the EC50 of 0.4 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human RNF43 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human RNF43 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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