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Conditions of optimal recombinant Human R spondin 1/RSPO1 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
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BP005579: Human R spondin 1/RSPO1 Protein with the C-His Tag
Source: The recombinant Human R spondin 1/RSPO1 Protein, (amino acids Arg31-Ala263) fused with a His tag at the C-terminus, was produced in CHO cells.
Molecular Weight:The recombinant Human R spondin 1/RSPO1 protein with the His tag at the C-terminus has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human R spondin 1 protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-R spondin 1 Antibody protein with the C-hFc tag with the EC50 of 2.9 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human R spondin 1/RSPO1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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