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Human P-Selectin ELISA Kit

GMP-140 ELISA Kit, CD62 ELISA Kit, Selectin P ELISA Kit

Catalog No. Product Name Size List Price (US$) Quantity
EK000036-EK0505-1 Human P-Selectin ELISA Kit 1 plate, 96T/plate 369.00
EK000036-EK0505-4 Human P-Selectin ELISA Kit 4 plates, 96T/plate 1328.00
Description

EK000036-EK0505: Human P-Selectin ELISA Kit

Range 62.5pg/ml-4000pg/ml
Sensitivity < 5 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human P-Selectin in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.

Principle

The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.

Reference

1. Herrmann, S.-M.; Ricard, S.; Nicaud, V.; Mallet, C.; Evans, A.; Ruidavets, J.-B.; Arveiler, D.; Luc, G.; Cambien, F. The P-selectin gene is highly polymorphic: reduced frequency of the pro715 allele carriers in patients with myocardial infarction. Hum. Molec. Genet. 7: 1277-1284, 1998.
2. Florian, V.; Schluter, T.; Bohnensack, R. A new member of the sorting nexin family interacts with the C-terminus of P-selectin. Biochem. Biophys. Res. Commun. 281: 1045-1050, 2001.
3. Burger, P. C.; Wagner, D. D. Platelet P-selectin facilitates atherosclerotic lesion development. Blood 101: 2661-2666, 2003.
4. Aigner, S.; Sthoeger, ZM.; Fogel, M.; Weber E.; Zarn, J.; Ruppert, M.; Zeller, Y.; Vestweber, D.; Stahel, R.; Sammar, M.; Altevogt, P. CD24, a mucin-type glycoprotein, is a ligand for P-selectin on human tumor cells. Blood. 1997 May 1;89(9):3385-95.

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