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EK000138-EK0810: Human Mer ELISA Kit
Range 0.312ng/ml-20ng/ml
Sensitivity <10 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human Mer in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)
Principle
The human Mer ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Mer specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Mer amount of sample captured in plate.
Reference
1. Graham DK, Dawson TL, Mullaney DL, Snodgrass HR, Earp HS (Oct 1994). "Cloning and mRNA expression analysis of a novel human proto-oncogene, c-mer". Cell Growth Differ 5 (6): 647?7. PMID 8086340.
2. Weier HU, Fung J, Lersch RA (Jun 1999). "Assignment of proto-oncogene MERTK (a.k.a. c-mer) to human chromosome 2q14.1 by in situ hybridization". Cytogenetic Cell Genet 84 (1?): 91?. doi:10.1159/000015223. PMID 10343112.
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