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EK000127-EK0782: Human IL-1RA ELISA Kit
Range 31.2pg/ml-2000pg/ml
Sensitivity < 2pg/ml
Specificity: No detectable cross-reactivity with any other protein.
Application: For quantitative detection of human IL-1RA in tissue lysates or cell culture supernates. The significance of detection in sera, plasma, body fluids is undetermined.
Expiration: Four months at 4°C and eight months at -20°C.
Background
The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene.1.2The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.4
Principle
IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL-1RA specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.
Reference
1Steinkasserer A, Spurr NK, Cox S, Jeggo P, Sim RB (July 1992). "The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14-q21, in the region of the IL-1 alpha and IL-1 beta loci". Genomics 13 (3): 654.
2?Patterson D, Jones C, Hart I, Bleskan J, Berger R, Geyer D, Eisenberg SP, Smith MF, Arend WP (January 1993). "The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region". Genomics 15 (1): 173.
3Patterson, D., Jones, C., Hart, I., Bleskan, J., Berger, R., Geyer, D., Eisenberg, S. P., Smith, M. F., Jr., Arend, W. P. The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region. Genomics 15: 173-176, 1993.
4?"Entrez Gene: IL1RN interleukin 1 receptor antagonist".
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