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EK000124-EK0881: Human HSP27 ELISA Kit
Range 62.5pg/ml-4000pg/ml
Sensitivity < 5pg/ml
Specificity: No detectable cross-reactivity with any other HSP .
Application: For quantitative detection of human HSP27 in sera, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. Hickey et al. (1986) determined that the HSP27 gene has 3 exons. Hunt et al. (1997) mapped the mouse Hsp25 gene to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.
Principle
HSP27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human HSP27 specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HSP27 amount of sample captured in plate
Reference
1. Hickey, E., Brandon, S. E., Potter, R., Stein, G., Stein, J., Weber, L. A. Sequence and organization of genes encoding the human 27 kDa heat shock protein. Nucleic Acids Res. 14: 4127-4145, 1986. (Note: Erratum: Nucleic Acids Res. 14: 8230 only, 1986.)
2. Hunt, C. R., Goswami, P. C., Kozak, C. A. Assignment of the mouse Hsp25 and Hsp105 genes to
the distal region of chromosome 5 by linkage analysis. Genomics 45: 462-463, 1997.
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