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Conditions of optimal recombinant Human HLA-A*24:02&B2M&MAGE-A3 (IMPKAGLLI) Tetramer Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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BP003793: Human HLA-A*24:02&B2M&MAGE-A3 (IMPKAGLLI) Tetramer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*24:02&B2M&MAGE-A3 (IMPKAGLLI) Tetramer Protein, (amino acids Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M)and IMPKAGLLI peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human HLA-A*24:02&B2M&MAGE-A3 (IMPKAGLLI) Tetramer protein with the His-Avi tag at the C-terminus has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HLA-A*24:02&B2M&MAGE-A3 (IMPKAGLLI) Tetramer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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