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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal recombinant Human HGF Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP004703: Human HGF Protein with the C-His Tag
Source: The recombinant Human HGF Protein, (amino acids Gln32-Ser728) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The recombinant Human HGF protein with the His tag at the C-terminus has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human HGF protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HGF R protein with the C-hFc tag with the EC50 of 9.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP004702: Human HGF Protein with the C-hFc Tag
Source: The recombinant Human HGF Protein, (amino acids Gln32-Ser728) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. The recombinant Human HGF protein with the hFc tag at the C-terminus has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human HGF R protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HGF protein with the C-hFc tag with the EC50 of 26.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Human HGF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following HGF Protein :
HGF Protein
Human HGF Protein
Biotinylated Human HGF R Protein
Cynomolgus HGF R Protein
Human HGF R Protein
Mouse HGF R Protein
Cynomolgus HGFA Protein
HGF A Protein
Human HGFA Protein
Mouse HGFA Protein
HGF B Protein
Syd Labs provides the following HGF Antibody :
HGF Antibody
Syd Labs provides the following HGF ELISA Kit :
Human HGF ELISA Kit
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