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Human GITR Ligand Protein

TNFSF18; AITRL; TL6; GITRL; GITR Ligand

Catalog No. Product Name Size List Price (US$) Quantity
BP005024 Human GITR Ligand/TNFSF18 Trimer Protein with the N-monomeric hFc Tag 100UG 315.00
BP005024 Human GITR Ligand/TNFSF18 Trimer Protein with the N-monomeric hFc Tag 500UG 1050.00
BP005599 Human GITR Ligand/TNFSF18 Trimer Protein with the N-His-Flag Tag 100UG 270.00
BP005599 Human GITR Ligand/TNFSF18 Trimer Protein with the N-His-Flag Tag 500UG 900.00
Description

BP005024: Human GITR Ligand/TNFSF18 Trimer Protein with the N-monomeric hFc Tag

Source: The recombinant Human GITR Ligand/TNFSF18 Trimer Protein, (amino acids Gln50-Ser177) fused with a monomeric hFc tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human GITR Ligand/TNFSF18 Trimer protein with the monomeric hFc tag at the N-terminus has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.
Activity: The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human GITR Ligand/TNFSF18 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005599: Human GITR Ligand/TNFSF18 Trimer Protein with the N-His-Flag Tag

Source: The recombinant Human GITR Ligand/TNFSF18 Trimer Protein, (amino acids Gln50-Ser177) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human GITR Ligand/TNFSF18 Trimer protein with the His-Flag tag at the N-terminus has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human GITR Ligand Trimer protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human GITR protein with the N-hFc tag with the EC50 of 0.44 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human GITR Ligand/TNFSF18 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human GITR Ligand/TNFSF18 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following GITR Protein :
Biotinylated Human GITR Protein
Cynomolgus GITR Protein
Human GITR Protein
Mouse GITR Protein

Syd Labs provides the following GITR Ligand Protein :
Biotinylated Human GITR Ligand Protein
Cynomolgus GITR Ligand Protein
Human GITR Ligand Protein
Mouse GITR Ligand Protein

Syd Labs provides the following GITR Antibodies :
Anti-Mouse GITR Monoclonal Antibodies

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