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Conditions of optimal recombinant Human Coagulation Factor III/Tissue Factor Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP003880: Human Coagulation Factor III/Tissue Factor Protein with the C-His Tag
Source: The recombinant Human Coagulation Factor III/Tissue Factor Protein, (amino acids Ser33-Glu251) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Coagulation Factor III/Tissue Factor protein with the His tag at the C-terminus has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human Coagulation Factor III protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Coagulation Factor III Antibody protein with the C-hFc tag with the EC50 of 4.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Coagulation Factor III/Tissue Factor Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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