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Home > Antibodies > A-Z Primary Antibodies > Human CD117 Protein

Human CD117 Protein

SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT

Catalog No. Product Name Size List Price (US$) Quantity
BP005099 Human CD117 Protein with the C-His Tag 100UG 240.00
BP005099 Human CD117 Protein with the C-His Tag 500UG 800.00
BP003460 Human CD117 Protein with the C-hFc Tag 100UG 255.00
BP003460 Human CD117 Protein with the C-hFc Tag 500UG 850.00
Description

BP005099: Human CD117 Protein with the C-His Tag

Source: The recombinant Human CD117 Protein, (amino acids Gln26-Thr516) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human CD117 protein with the His tag at the C-terminus has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human SCF at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human CD117 protein with the C-His tag with the EC50 of 1.6 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD117 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003460: Human CD117 Protein with the C-hFc Tag

Source: The recombinant Human CD117 Protein, (amino acids Gln26-Pro520) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human CD117 protein with the hFc tag at the C-terminus has a predicted MW of 82.46 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human SCF protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human CD117 protein with the C-hFc tag with the EC50 of 12.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD117 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human CD117 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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